Male Wistar rats were randomly allocated to 5 groups containing 8 animals each. Group I (Normal Control) and Group II (Model Control) animals received distilled water throught out study period. Group III, Group IV and Group V received Ascorbic acid (250 mg/kg), Methanolic extract of Trigonella foenum graecum Linn (MTfg) 300 mg/kg and MTfg 600 mg/kg respectively once a day per oral for 28 days. On the 27th day MI was induced in animals of Group II to Group V by administration of the first dose of ISO (100 mg/kg, S.C) followed by second dose of ISO after 24 hours. 12 hours after the second dose of ISO, rats were anaesthetized and decapitated. Blood was collected from each animal. Serum was obtained by centrifugation at 4000 rpm for 15 min and at the same time heart was rinsed with ice-chilled physiologic saline and weighed. It was then used for the estimation of various biochemical parameters. Administration of Isoproterenol significant decreased Na/k ATPase, K, MDA and increased CK-MB, LDH, Ca ATPase, Mg ATPase, Na, GSH, SOD, Catalase. While on ECG ST Segment elevation and heart rate were seen in Myocardial Infarction. These changes were significantly prevented by treatment with Ascorbic acid and MTfg. Cardioprotective activity of MTfg can be attributed to decrease Cardiac Marker Enzyme level and normalizes ECG changes.
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